© 2020 Laboratory Corporation of America® Holdings. Retrospective assessment of the clinical relevance of surgical biopsies of abdominal lymph nodes in cats: 51 cases (2014–2018). Histopathology, immunohistochemistry, and molecular clonality testing are metrics frequently used to diagnose chronic enteropathy (CE) in cats. Additionally, a serum feline immunodeficiency virus antibody test and feline leukemia virus antigen test were performed if the status of the cat was unknown (n = 4). All authors are either employed by (Marsilio, Lidbury, Suchodolski, and Steiner) or affiliated with (Ackermann) the Gastrointestinal Laboratory at Texas A&M University, which offers laboratory tests, including histopathology services, on a fee‐for‐service basis. One possible explanation for the frequent finding of clonal rearrangements would be the presence of malignant but indolent lymphocyte clones. Histopathology is an international journal intended to be of practical value to surgical and diagnostic histopathologists, and to investigators of human disease who employ histopathological methods. A physical examination was performed by a single board‐certified internist (SM). Cats had a median age of 9.5 years (range, 3‐18 years), median body weight of 5.0 kg (range, 2.6‐10.8 kg), and median body condition score of 6 out of 9 (range, 5‐9). as ‘Trucut Needle’. Turnaround time is defined as the usual number of days from the date of pickup of a specimen for Morphologic changes were present in 19 cats. In addition, clinical signs subsided after treatment with a hypoallergenic diet. Statistical significance was set at P < .05. Entire specimen; paraffin embedded (FFPE) tissue block(s) or slide(s) sectioned from FFPE tissue block(s) at 4-5 microns, Jars of assorted size containing 10% buffered formalin. Histopathologic evaluation of H&E‐stained gastric biopsy sections had abnormalities in all 18 available cats. This finding was reported to be minimal in 4, minimal to mild in 2, mild in 5, mild to moderate in 5, and moderate in 2 cats. Another cat developed clinical signs of CE with severe vomiting approximately 17 months post‐endoscopy. Malignancy during and after the treatment. Findings were reported descriptively, and numerically scored according to the WSAVA histopathologic scoring system.1, 13 Briefly, morphological features (eg, surface epithelial injury, crypt hyperplasia, crypt dilatation or distortion, and fibrosis or atrophy) and inflammatory changes (eg, lamina propria lymphocytes, plasma cells, eosinophils, neutrophils, and macrophages) were assessed histologically and assigned a score (normal = 0, mild = 1, moderate = 2, and marked = 3).1. Since the introduction of clonality assays for the diagnosis of intestinal T cell lymphoma in cats in 2005, several studies have investigated the value of PCR‐based clonality assays in the diagnosis of intestinal and extraintestinal T cell lymphoma in cats.9, 12, 31, 32 Subsequently, clonality assays have become the gold standard for the diagnosis and differentiation of lymphoma in cats.33 However, similar to the WSAVA criteria, PARR for the molecular diagnosis of intestinal T cell lymphoma in cats was developed based on samples obtained from healthy young (ie, 12‐18 months old) SPF colony cats and thus might not be representative of the target population. Again, this would appear to be a reasonable explanation for the 2 cats that were diagnosed with SCL on histopathology and much later developed clinical signs of CE. and you may need to create a new Wiley Online Library account. Our results suggest that histological scoring criteria may need to be revised and adapted to a more adequate reference population. One cat had an increased fPLI concentration (15.6 μg/L; reference interval, ≤3.5 μg/L) without any current or prior associated clinical signs. Both cats were diagnosed previously with SCL based on the first histopathologic examination as well as laboratory results on H&E, immunohistochemistry, and PARR. This cat did not receive any treatment. examine it. However, the target DNA is the DNA that is amplified during the PCR (ie, DNA from T cells in TRG clonality assays). With low numbers of lesional T cells, the ratio of target DNA to total DNA decreases and preferential amplification and pseudoclonality may occur despite adequate total DNA concentration and purity.11, 26 This is an important reason that clonality assays should always be interpreted in the context of histopathology and immunohistochemistry, and thus should be performed in the same laboratory, preferably by the same pathologist. Follow‐up data were available for all 20 cats. Preparation for Histopathology - Core / Trucut Biopsy Test, Uses of Histopathology - Core / Trucut Biopsy Test, Procedure for Histopathology - Core / Trucut Biopsy Test, Price for Histopathology - Core / Trucut Biopsy Test. provides the pathologist with a core of undamaged tissue from the Tumor/ This prospective study was conducted at the Veterinary Medical Teaching Hospital, Texas A&M University. Results from the H&E‐based histopathology, immunohistochemistry, and molecular clonality analysis were integrated and reported by the external pathologist. Testing schedules may vary. In 2 cats, a diagnosis of SCL was made based on histopathology. Availability of Results Tissue specimens were collected from the stomach and duodenum and evaluated single blinded by a board‐certified pathologist. To describe results of histopathology, immunohistochemistry, and clonality testing of endoscopically‐derived biopsy specimens of the upper small intestinal tract from a cohort of clinically healthy client‐owned cats. According to the owner, 1 cat had a short episode of acute self‐limiting diarrhea within the 6 months before the study. Neither cat was available for postmortem examination. Intestinal biopsy specimens from clinically healthy client‐owned cats commonly had abnormal findings on histopathology, immunohistochemistry, clonality testing, or some combination of these without apparent clinical relevance. A detailed list of the WSAVA scores is shown in Supporting Information Table S2. Lesions were interpreted as consistent with lymphocytic enteritis with mild epitheliotropism in the upper SI tract and lymphocytic enteritis within the lower SI tract. Results from H&E stains, immunohistochemistry, and PARR were integrated by the external pathologists and reported as case interpretations.

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